Here, we introduce RBPseg, a method that combines monomeric ESMFold predictions with a structural- based domain identification approach, to divide tail fiber sequences into manageable fractions for high- confidence modeling with AF2M. FC-SC Type: Circular to square tail fiber, where FC connects to ODF boxes, and SC connects to equipment ports. Widely used in early SBS and Optix devices. SC-SC Type: Known as square to. Recent advancements in deep learning-based protein structure prediction, such as AlphaFold2-multimer (AF2M) and ESMfold, allow for the generation of high-confidence predicted models of complete tail fibers. Understanding these structures is key to grasping phage behavior. These segments are then predicted in parallel using AF2M and assembled into a 26 full fiber model.
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